TiO2 has excellent photocatalytic properties and several studies have reported the increase in its specific surface area. The structure of TiO2 nanofibers indicates promising improved photocatalytic properties and these nanofibers can thus potentially be applied in air pollution sensors and pollutant removal filters. In this study, a TiO2 nanofiber was fabricated by the electrospinning method. The fabrication processing factors such as the applied voltage, the distance between nozzle and collector, and the inflow rate of solution were controlled. The precursor was titanium (Ⅳ) isopropoxide and as-spun TiO2 nanofibers were heated at 450℃ for 2 h to obtain an anatase crystalline structure. The microstructure was analyzed using field emission scanning electron microscope (FE-SEM) and X-ray diffraction analysis (XRD). The anatase phase was observed in the TiO2 nanofibers after heat treatment. The diameter of TiO2 nanofibers increased with the flow rate, but decreased with decreasing applied voltage and nozzle to collector distance. The diameter of TiO2 nanofibers was controlled in the range of 364 nm to 660 nm. These nanofibers are expected to be very useful in photocatalytic applications.