Climate conditions, especially transport and storage, are a very important factor in the process of sampling and testing insulation oil in the field. The samples of insulating oil exposed to the atmosphere affect the dielectric strength, total acid number and moisture test value by oxygen and high humidity environment and may also affect the results according to the criteria specified in each test. Therefore, reliable test values for insulating oil testing require consideration of the atmospheric environment of the test site, including oxygen and humidity. In this paper, each test was conducted on insulating oil exposed to various time and humidity environments, and the effect of the atmospheric environment on the test results was analyzed by comparing and analyzing with the first insulating oil.