In this study, the application of biosensor having a large surface area for more effective and AAO (anomic aluminium oxide) template in order to gain concentration and voltage of anodizing process morphology changes to the control of experiments were conducted. The biosensor surface may increase the response characteristics by having a large surface area. So the entrance to a little more efficient wide depth sensing experiment was carried out to obtain a structure body with a branch shape with a large surface area with increasing. Experimental results from the FE-SEM observation was obtained template morphology. As a result, depending on the anodizing time, the depth of the layer of aluminum oxide was found that it was confirmed that the deepening of the pore size changes according to anodizing condition. And measuring the detection performance according to the conditions in the electrolyte and the reaction because of blood using a biosensor measuring sensing property according to the depth of the pore depth is considered that does not have a significant impact.

We fabricated the electrolyte-dielectric-metal (EDM) sensor on the base of AAO (anodicaluminum oxide) template with variation of the anodizing temperature. When a surface is immersed orcreated in an aqueous solution, a discontinuity is formed at the interface where such physicochemicalvariables as electrical potential and electrolyte concentration change significantly from the aqueous phaseto another phase. Because of the different chemical potentials between the two phases, charge separationoften occurs at the interfacial region [1]. This interfacial region, togeter with the charged surface, isusually known as the electrical double layer (EDL) [2]. The structural and electrochemical properties ofAAO sensor were investigated for applications in capacitive pH sensors. To change the thickness of theAAO template, the anodizing temperature was varied from 5℃ to 20℃, the thickness of the AAOtemplate invreased from 300 nm to 477 nm. The pH sensitivity of sensors with the anodizing temperatureof 20℃ showed the highest value of 56.4 mV/pH in the pH range of 3 to 11. The EDM sensor with theanodizing temperature of 20℃ exhibited the best long-term stability of 0.037 mV/h.